Evaluation of antibacterial activity of Vachellia nilotica (L) PJH Hurter and Mabb pods powder aqueous extract on multidrug-resistant bacteria, phytochemical screening and quality control

Abstract

Nowadays, the proliferation of germs resistant to classic antibiotics explains the urgency discovery of new antibiotics molecules. The objective of this study was to evaluate the antibacterial activity of aqueous extracts of eight (8) samples of Vachellia nilotica pods powders collected in the city of Ouagadougou on multiresistant bacteria, to assess their health quality and study the phytochemical composition. The disk diffusion method was used for the study of antibacterial activity, that of complexation and spectrophotometric activity were used for phytochemical characterization. The sanitary quality of vegetable powders has been assessed by research into pathogenic microorganisms. All samples showed diameters of inhibition on test germs. The highest diameter was obtained with the sample of 1200 Logements, 17 ± 1.7 mm on Shigella dysenteria, 14.67 ± 0.50 mm on Salmonella typhimurium, 14 mm on Staphylococcus aureus and 15 mm on Bacillus cereus. The phytochemical study revealed the presence of alkaloids, tannins, glycosides, sterols and polyphenol in the aqueous extracts of the plant. The results of the microbiological analyzes showed that the powder samples were of unsatisfactory quality. However, there is a virtual absence of coliforms and enterobacteria.