Study of the physicochemical and toxicological profiles of three products commonly used in vaginal practices among women in the city of Ouagadougou, Burkina Faso.
DOI:
https://doi.org/10.64707/revstss.v48i2.2000Keywords:
Key Words: Acute toxicity; Irritation potential; Vaginal practices; Helth risk; Burkina Faso.Abstract
Vaginal practices encompass various methods of hygiene and other interventions, ranging from external cleansing with water or mild products to internal washing (douching) and the insertion of substances for hygienic, sanitary, or sexual reasons. Due to the wide variety of products and methods of use, these practices may carry certain risks. The aim of this study was to characterize the physicochemical and toxicological profile of three recipes used by women in Ouagadougou for vaginal practices. These recipes are: Mint Crystals, Petit Bandit, and Djeka. The phytochemical profile of the three recipes was analyzed using thin-layer chromatography. Acute toxicity and eye and skin irritation were determined based on the guidelines of the Organization for Economic Cooperation and Development (OECD). In solution, the pH of the recipes was 3.58 ± 0.02, 6.86 ± 0.34, and 6.22 ± 0.06 for Djeka, Petit Bandit, and Mint Crystals, respectively. Djeka contains flavonoids, tannins, phenolic compounds, and coumarins. Mint Crystals contain sterols and terpenes. No chemical groups were identified in Petit Bandit tablets. The acute toxicity study showed that each of the three formulations has an estimated LD50 of 5000 mg/kg bw. Mint Crystals and Petit Bandits, at a single dose of 2000 mg/kg bw, caused a significant decrease in the relative weight of the liver, kidneys, and spleen of the test mice. Djeka and Petits Bandits have minimal eye irritation potential, and Mint Crystals has moderate irritation potential. All three recipes are non-irritating to the skin. The results are preliminary, but they raise questions about the safety of misuse of the recipes.
References
References
Brahmi, F., Kampemba Mujinga, F., Guendouze, N., Madani, K., Boulekbache, L., & Duez, P. (2025). Benefits of Traditional Medicinal Plants to African Women’s Health: An Overview of the Literature. Diseases, 13(5), 160. https://doi.org/10.3390/diseases13050160
Dori, D., Porgo, R., Zamané, H., Méda, N. and Semdé, R. (2024) Study of Harmful Vaginal Practices of Women in the Municipality of Ouaga dougou, Burkina Faso. Health, 16, 425-438. https://doi.org/10.4236/health.2024.165030
Ed-Dahmani I, Kara M, Lfitat A, Touijer H, Bousraf FZ, Slighoua M, Nouioura G, El Atki Y, El Fadili M, Taleb M, Abdellaoui A. Evaluation of Polyphenolic Content, Antioxidant and Antimicrobial Activities, and Toxicity Study of Ferula communis L. FruitsTrop J Nat Prod Res. 2024; 8(2):6423-6432. http://www.doi.org/10.26538/tjnpr/v8i2.34
Erdogan A. and Bechelaghem N. (2018): To ‘douche’ or not to ‘douche’: hygiene habits may have detrimental effects on vaginal microbiota, Journal of Obstetrics and Gynaecology, DOI: 10.1080/01443615.2017.1395398
ff10.4000/anthropodev.2153ff. ffinserm-04940017f
Gnahoué G, Koné A, Kouadio KJ, Koffi K. Assessment of the acute, subacute and subchronic toxicity of a total aqueous extract of leaves of Alchornea cordifolia (Schumach and Thonn) Müll Arg (Euphorbiaceae) on rats Wistar. J Pharmacogn Phytochem. 2021; 10(1): 1609 14.
Holdcroft AM, Ireland DJ, Payne MS. The Vaginal Microbiome in Health and Disease-What Role Do Common Intimate Hygiene Practices Play? Microorganisms. 2023 Jan 23;11(2):298. doi: 10.3390/microorganisms11020298. PMID: 36838262; PMCID: PMC9959050.
Jazy MA, Sanogo RMH. Chromatographie sur couche mince et activité antiradicalaire d’extraits de Pupalia Lappacea (L.) Juss. Amaranthaceae. ESJ. 2018; 14(3):17.
Leo VD, Benvenuti C (2015) Pharmacological, Microbiological and Clinical Activity of Feminine Intimate Cleansers Based on Plant Extracts Active Principles (Saugella Line). J Women’s Health Care 4: 244. doi:10.4172/2167- 0420.1000244
Lin, Y.-P.; Chen, W.-C.; Cheng, C.-M.; Shen, C.-J. Vaginal pH Value for Clinical Diagnosis and Treatment of Common Vaginitis. Diagnostics 2021, 11, 1996. https:// doi.org/10.3390/diagnostics11111996
Mininel F. La circulation transnationale des produits vaginaux en Afrique de l’Ouest. Des objets de plaisir, séduction et envoûtement. Anthropologie et développement, 2023, 54, pp.89 - 106.
Naidu M, Sultan AH, Thakar R. Reducing obstetric anal sphincter injuries using perineal support: our preliminary experience. Int Urogynecol J. 2017 Mar;28(3):381-389. doi: 10.1007/s00192-016-3176-4. Epub 2016 Oct 19. PMID: 27761586.
Nnanga, N., Ngolsou, F., Soppo Lobe, V., Maniépie, N., Nyangono Ndongo, M. et al. (2020). Identification des Composés Bioactifs Pouvant Justifier l’Usage des Feuilles de Psychotria Calceata en Médecine Traditionnelle au Cameroun. HEALTH SCIENCES AND DISEASE, 21(9). https://doi.org/10.5281/hsd.v21i9.2272
OCDE (2002), Essai n° 423: Toxicité orale aiguë - Méthode par classe de toxicité aiguë, Lignes directrices de l'OCDE pour les essais de produits chimiques, Section 4, Éditions OCDE, Paris, https://doi.org/10.1787/9789264071018-fr.
OCDE (2015), Essai n° 404 : Effet irritant/corrosif aigu sur la peau, Lignes directrices de l'OCDE pour les essais de produits chimiques, Section 4, Éditions OCDE, Paris, https://doi.org/10.1787/9789264242685-fr.
OCDE (2023), Essai n° 405 : Effet irritant/corrosif aigu sur les yeux, Lignes directrices de l'OCDE pour les essais de produits chimiques, Section 4, Éditions OCDE, Paris, https://doi.org/10.1787/9789264185548-fr.
Ouédraogo J, Ilboudo S, Ouédraogo GG, Dakuyo V, Ouédraogo S, Somda GD, Ouédraogo JCRP, Ouédraogo M, Semdé R, Ouédraogo S (2025). Impacts of SAYE PLUS, an antimalarial phytomedicine with potential anti-COVID-19, on the physical, biological, and genotoxicity parameters of rodents in short-term toxicity studies. J Toxicol. 2025 Jul 21;2025:7536185. doi: 10.1155/jt/7536185.
Ouédraogo S., Traoré S., Ouédraogo J.C. W., Koala M, Belemnaba L., Ouédraogo N. et al. Quality control and standardization Of faca ® syrup. International Journal of Drug Delivery 9 (2017) 101-106 http://www.arjournals.org/index.php/ijdd/index
Roger KG. CCM d’extraits sélectifs de 10 plantes utilisées dans le traitement traditionnel du cancer du sein en Côte d’Ivoire. European Journal of Scientific Research. 2011; 63(04): 592 603.
Semdé, 2023. Etude physico-chimique et toxicologique des trois recettes les plus utilisées comme « secrets de femmes » dans la ville de Ouagadougou, au Burkina Faso. Thèse d’Exercice en Médecine. Université Joseph KI-ZERBO, Ouagadougou. Soutenue le 31 mars 2023. 150pp.
Traoré M. Alchornea cordifolia. Schmach (Euphorbiaceae) : Revue des activités biologiques et de l’étude de la toxicologie. Université de Bamako. 2005.
United Nations. 2023. Globally harmonized system of classification and labelling of chemicals (GHS). Tenth revised edition. New York and Geneva: United Nations; 586 p.
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