Electrophoresis confirmation of SS and non-sickle cell subjects using realtime PCR in Burkina Faso

Authors

  • Marie Simone TRAORE 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Pegdwendé Abel SORGHO 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Abdoul Karim OUATTARA 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Paul OUEDRAOGO3 3.Hôpital Saint Camille de Ouagadougou (HOSCO), 09 BP 444 Ouagadougou 09, Burkina Faso
  • Albert Théophane YONLI 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Hermann SOMBIÉ 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Abdou Azaque ZOURE 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso 4.Laboratoire de Recherche Biomédicale (LaReBio), Département Biomédical et Santé Publique, Institut de Recherche en Sciences de la Santé (IRSS/CNRST), 03 BP 7192 Ouaga03, Burkina Faso
  • Théodora M. ZOHONCON 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso 3.Hôpital Saint Camille de Ouagadougou (HOSCO), 09 BP 444 Ouagadougou 09, Burkina Faso 5.Faculté de Médicine, Université Saint Thomas d'Aquin (USTA), 06 BP 10212 Ouagadougou 06, Burkina Faso.
  • Djénéba OUERMI 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso
  • Jacques SIMPORE 1.Laboratoire de Biologie Moléculaire et Génétique (LABIOGENE), Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso 2.Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA), 01 BP 364 Ouagadougou 01, Burkina Faso 3.Hôpital Saint Camille de Ouagadougou (HOSCO), 09 BP 444 Ouagadougou 09, Burkina Faso

Keywords:

sickle cell disease, hemoglobinopathy, cellulose acetate electrophoresis, real-time PCR, Burkina Faso

Abstract

Sickle cell disease, a genetic disease that is widespread in the world, has a high prevalence in Burkina Faso. The
aim of this study was to confirm the electrophoresis of SS and non-sickle cell subjects by real-time PCR in order
to propose it as an early diagnosis method for hemoglobinopathies.
A total of 280 patients participated in this study, including 86 sickle aged 1 to 15 years (non-pregnant) cell patients
and 194 pregnant women with unknown hemoglobin genotypes and no HbSS pregnant women was detected after Hb genotype analysis. All patients underwent hemoglobin electrophoresis (EHb) and PCR tests to determine their
genotypes. In addition, those with SS hemoglobin performed serum iron, ferritin, and hemogram tests.
The sickle cell group consisted of 41.9% women and 58.1% men. PCR genotyping in pregnant women yielded the
following genotype frequencies: AA (82.4%); AC (9.3%); AS (5.2%); CC (3.1%). All EHb tests were 100%
confirmed by PCR.
PCR was 100% sensitive and specific compared to EHb tests. PCR, which allows for prenatal and perinatal testing,
also differentiates hemoglobin S from hemoglobin D, and hemoglobin C from hemoglobin A2. Therefore, the PCR
test for hemoglobin can now be recommended for routine exams of hemoglobinopathies.

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Published

2024-04-25

How to Cite

TRAORE, M. S. ., SORGHO, P. A., OUATTARA, A. K. ., OUEDRAOGO3, P. ., YONLI, A. T. ., SOMBIÉ, H. ., ZOURE, A. A. ., ZOHONCON, T. M. ., OUERMI, D. ., & SIMPORE, J. . (2024). Electrophoresis confirmation of SS and non-sickle cell subjects using realtime PCR in Burkina Faso. Sciences De La Santé, 44(2), 51–61. Retrieved from https://revuesciences-techniquesburkina.org/index.php/sciences_de_la_sante/article/view/1074

Issue

Vol. 44 No. 2 (2021): juillet - décembre

Section

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